Part:BBa_K1313001:Design

Oxoacyl Reductase

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 625
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 733
  Illegal AgeI site found at 133
  Illegal AgeI site found at 277
  
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

Codon optimization for a better expression on E.coli.

Source

ATGAAACGCCTGGTCTGTGTTCTGCTGGTCTGCTCGTCTGCTGTTGCTCCGCTGCATCATAGCCTGGTTGATCAC CCGTCTGCCCATCCGGGTCATGGCGGTGGCCCTGTTAGTGCGGGCGTCGCCCTGGTGACCGGTGGCTCCCGTGGC GTTGGTAAAGGCGTCGCAACGGCACTGGGTGCGGCGGGTTGGACCGTGTATGTTACGGGTCGTGGCCCGATGACC GAAGATTGCCCGCTGAGTGCATCCGCTCGTACCGTTACGGGTGCGGGTGGCACCGGTATTGCAGTCGCCTGTGAC CACCGTGAAGATGCAGACGTGCGTGCACTGTTTGCTCGCATCAGTGATGAACAGGCCGGTCGCCTGGACCTGCTG GTTAACAATGTCTGGGCAGCTCCGAAAGGCTTTGGTGGCTTCAGCACCCCGTTCTGGGAACGTCCGCTGGATGAC TGGGATTCTCTGATTGGTGTTGGCCTGCGTGCGCATTATGTGGCCTCAGTTGAAGCAGCACGTCTGATGGTGCCG CGTCGCTCGGGTCTGATTGCGAACATCAGCTCTTTTGGTACCCGCGGCCATCTGCACTCAGTGCTGTACGGCATG TCGAAAGCAGGCCTGGATAAAATGGCTAGCGACATGGCAGCTGAACTGGCCCCGCACGATGTCCGTGCAGTGAGT CTGTGGCCGGGCATTGTGCGCACCGAAATGATCGTTGCGTCCGGTCTGGAAACGATCGCCGGCTTTAGCGTTGCA GATGCTGAATCTCCGGAATTTATTGGTGAAGTCATCTGCGCACTGACCGCCGACCCGACGCTGCCGCAGCGTTCT GGTCATACCTTCGTCACGGCAGAACTGGCTGTGGAATACGGTATTACCGATACGCAAGGTCGTACCCCGCCGAGC CACCGTGAACCGTTTGGTGGCGGTCCGCTGTTCAATATCACG